Part:BBa_K5101007
Certolizumab (Anti-TNF-α )Heavy Chain With OmpA
Anti-TNF-α is a non-glycosylated monovalent Fab fragment of TNF-α monoclonal antibody with a molecular mass of 47.33 kDa. The Fab fragment is composed of a light chain (VL, CL domain) and a heavy chain (VH, CH1 domain) connected near the hinge region by disulfide bonds, with the light chain consisting of 214 amino acid residues and the heavy chain consisting of 229 amino acid residues.Each MCS is preceded by a T7 promoter, lac operator, and a ribosome binding site (RBS). To ensure that Anti-TNF-α could be successfully assembled, we added ompA signal peptide sequence before the N-terminal end of the amino acid sequence of each chain. ompA signal peptide sequence can guide the transfer of secreted proteins to the secretory pathway for periplasmic translocation.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 603
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 603
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 603
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 603
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 603
Illegal AgeI site found at 585 - 1000COMPATIBLE WITH RFC[1000]
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